Separation of pepsin I, pepsin II A, pepsin II B, and pepsin III from human gastric mucosa

Crystalline Pepsin Iii. Preparation of Active Crystalline Pepsin from Inactive Denatured Pepsin By

The results (1) of the experiments with crystalline pepsin isolated from crude pepsin preparations indicated that the material is a pure substance and that the proteolytic activity is a property of the protein molecule itself and is not due to the presence of a separate non-protein impurity. No indication of the presence of a more highly active nonprotein molecule was obtained in the solubility...

Studies of Gastric Pepsin. Ii. Secretion of Pepsin in Cases of Duodenal Ulcer and Pseudo-ulcer.

Few articles on concentration of pepsin in the gastric juice of patients with disease of the stomach and duodenum are to be found in recent literature, and the results reported by different observers are not always in accord. We shall consider here only the concentration of pepsin in the gastric juice of persons suffering with the syndrome of peptic ulcer. Wilcox (5), in 1908, reported that the...

Crystalline Pepsin

The decrease in protein nitrogen and in the activity of solutions of crystalline pepsin at pH 1.8 and 45 degrees C. has been determined. The decrease in activity, as measured with eleven different methods, is in exact proportion to the decrease of protein nitrogen of the solution. The measurements were continued until less than 5 per cent of the original protein remained. These results indicate...

The separation of fibrinogen from ;pepsin-inhibitor'.

Crystalline Pepsin v. Isolation of Crystalline Pepsin from Bovine Gastric Juice By

A crystalline protein having powerful proteolytic activity has been described in previous papers of this series (1). This protein was isolated from commercial pepsin preparations which, in turn, had been prepared from the gastric mucosa of swine, t The possibility exists that this protein was formed from some more complicated compound during the process of extraction and does not represent the ...